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By Dr. Jamie York

90 seconds. To help facilitate the extraction of CBD, samples were put on a shaker table for 5 minutes. Centrifugation was then performed for 5 minutes at 3000 rpm at 4 °C. The supernatant was transferred (50 µL) to a vial, and the solvent was dried down with a stream of nitrogen. Each sample was reconstituted with 25:75 water:acetonitrile (1 mL) and vortexed. A 300 µL aliquot was transferred to a 0.2 µm PTFE Thompson filter vial (cat.# 25893) and filtered prior to LC-UV analysis.


Cannabidiol is a nonpsychoactive cannabinoid found in cannabis plants and cannabis-related products. Currently, CBD is not an FDA scheduled drug in the United States, and it is sold in a number of different topical products, including lotions, balms, and creams. Developing extraction and analytical methods for these matrices can be very difficult because they are composed of a diverse array of ingredients, which makes it challenging to develop universal procedures that work for all samples. In the LC-UV method for CBD in topicals developed here, one sample preparation and method of analysis was successfully used to quantify CBD in all three sample types. The product of this sample preparation method is a clean extract that will ensure instrument uptime and maximize column lifetime while providing fast and reliable quantitation of CBD in these complex matrix samples.

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Table I: Advertised label value of CBD, experimentally calculated concentration of CBD, calculated precision, and percent difference (using Equation 1) compiled for each sample.